pGLO Observations , Data Recording & Analysis
1. Obtain your team plates. Observe your set of “+pGLO” plates under room light and with UV light. Record numbers of colonies and color of colonies. Fill in the table below.
2. What two new traits do your transformed bacteria have?
Our new, transformed bacteria, are now resistant to ampicillin, and they glow green (as shown in the picture above) in the prescence of arabinose sugars.
3. Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
4. What is the role of arabinose in the plates?
The arabinose is a sugar that triggers a fluroscent green dye in the bacteria that makes the bacteria glow green.
5. List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
Green fluorescent protein can be used to make transgenic pets. For example, in a french laboratory, Alba, a green-fluorescent rabbit was created using GFP. This could be used for to save animals, like rats, by making them easier to see, on roads and freeways.
1. Obtain your team plates. Observe your set of “+pGLO” plates under room light and with UV light. Record numbers of colonies and color of colonies. Fill in the table below.
|
Plate
|
Number of Colonies
|
Color of Colonies under Translucent Room
Light
|
Color of Colonies under UV Light
|
|
- pGLO LB
|
1 large colony
|
Gray
|
Gray
|
|
- pGLO LB/amp
|
0
|
N/A
|
N/A
|
|
+ pGLO LB/amp
|
Approximately 80
|
White
|
White
|
|
+ pGLO LB/amp/ara
|
Approximately 50
|
White
|
Green
|
2. What two new traits do your transformed bacteria have?
Our new, transformed bacteria, are now resistant to ampicillin, and they glow green (as shown in the picture above) in the prescence of arabinose sugars.
3. Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
An Escherichia coli cell (a single bacteria) has a volume of approximately 1 micrometer cubed. And there are 1,000,000,000 (1e9) cubic micrometers (e-coli cells) in one micro litter. Thus, if the plate is 100 micro liters, there are 1e9 * 100 bacteria on the plate.
There arer 100,000,000,000 - 100 trillion bacteria in the 100 micro liters on the plate.
4. What is the role of arabinose in the plates?
The arabinose is a sugar that triggers a fluroscent green dye in the bacteria that makes the bacteria glow green.
5. List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
Green fluorescent protein can be used to make transgenic pets. For example, in a french laboratory, Alba, a green-fluorescent rabbit was created using GFP. This could be used for to save animals, like rats, by making them easier to see, on roads and freeways.
GFP can also be sued for macro-photography. For example, the spread of virus infections can be tracked by using GFP. This could help us stop contagious diseases from spreading.
GFP can also serve as a reporter gene, which attaches to a sequence of another gene. GFP can be heritable, and thus allowing for long term studies.
6. Give an example of another application of genetic engineering.
One example of genetic engineering is how dragonflies are currently being genetically engineered to become cybernetic drones, for surveillance. This could be really helpful for intelligence gathering operations, and it also has other uses.
One example of genetic engineering is how dragonflies are currently being genetically engineered to become cybernetic drones, for surveillance. This could be really helpful for intelligence gathering operations, and it also has other uses.
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